Tryptophan Hydroxylase 1 抗体 (pSer260)
Quick Overview for Tryptophan Hydroxylase 1 抗体 (pSer260) (ABIN6658146)
抗原
See all Tryptophan Hydroxylase 1 (TPH1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer260
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Supplier Product No.
- 600-401-e49
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Supplier
- Rockland
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原理
- Tryptophan Hydroxylase phospho S260 Antibody
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交叉反应 （详细）
- Anti-Tryptophan Hydroxylase pS260 antibody is directed against rat Tryptophan Hydroxylase.
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纯化方法
- Tryptophan Hydroxylase pS260 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.
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免疫原
- Anti-Tryptophan Hydroxylase pS260 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 of Tryptophan Hydroxylase.
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亚型
- IgG
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anti-Tryptophan Hydroxylase 1 (TPH1) (AA 35-62), (N-Term) antibody
TPH1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB21510 unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 257-405) antibodyTPH1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (pSer58) antibodyTPH1 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 345-444) antibodyTPH1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (N-Term) antibodyTPH1 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 26-75) antibodyTPH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (AA 231-280) antibodyTPH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) antibodyTPH1 适用: 人 WB, IHC, IF/ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (pSer260) antibodyTPH1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tryptophan Hydroxylase 1 (TPH1) (pSer58) antibodyTPH1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optional[Neutralization_Dilution]: 1:1000
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说明
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Anti-Tryptophan Hydroxylase pS260 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of 55 kDa in size corresponding to tryptophan hydroxylase protein phosphorylated at Ser260 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- Tryptophan Hydroxylase 1 (TPH1)
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别名
- Tryptophan Hydroxylase
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背景
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Synonyms: Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH
Background: Tryptophan Hydroxylase pS260 antibody detects Tryptophan Hydroxylase, which catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH. Anti-THPpS260 Antibody is ideal for investigators involved in Cell Signaling, and Neuroscience research.
Gene Name: TPH1
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基因ID
- 24848
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UniProt
- P09810
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途径
- Feeding Behaviour
抗原
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