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- 抗原 See all DLG1 抗体
- DLG1 (Discs, Large Homolog 1 (DLG1))
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抗原表位
- N-Term
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DLG1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Anti-SAP97 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with SAP97 from Mouse, Human, and Rat based on 100% homology with the immunizing sequence. No cross-reactivity against PSD95, SAP102 and Chapsyn110 expressed in transfected cells. Cross-reactivity with SAP97 from other sources has not been determined. Ion Channels research.
- 免疫原
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Immunogen: SAP97 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an n-terminal region of rat SAP97.
Immunogen Type: Recombinant Protein
- 克隆位点
- S64-15
- 亚型
- IgG1
- Top Product
- Discover our top product DLG1 Primary Antibody
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anti-Discs, Large Homolog 1 (DLG1) antibody
DLG1 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 662-773) antibodyDLG1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-90) antibodyDLG1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1D8 unconjugated
anti-Discs, Large Homolog 1 (DLG1) (Internal Region) antibodyDLG1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-190) antibodyDLG1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 643-673) antibodyDLG1 适用: 人 WB 宿主: 兔 Polyclonal RB53955 unconjugated
anti-Discs, Large Homolog 1 (DLG1) (N-Term) antibodyDLG1 适用: 人, 大鼠, 小鼠, 犬 WB, IHC, IP, ICC, EM 宿主: 小鼠 Monoclonal RPI 197-4 unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-90) antibodyDLG1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D8 unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-90) antibodyDLG1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B12 unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-SAP97 Antibody is suitable for use in WB, IHC and IP. Expect a band approximately ~130 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: 1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- 抗原
- DLG1 (Discs, Large Homolog 1 (DLG1))
- 别名
- SAP97 (DLG1 产品)
- 别名
- DLG1 antibody, Dlgh1 antibody, SAP97 antibody, B130052P05Rik antibody, E-dlg/SAP97 antibody, SAP-97 antibody, mKIAA4187 antibody, DLGH1 antibody, dJ1061C18.1.1 antibody, hdlg antibody, discs large MAGUK scaffold protein 1 antibody, discs, large homolog 1 (Drosophila) antibody, DLG1 antibody, Dlg1 antibody
- 背景
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Synonyms: Dlgh1, SAP97, Dlg1, Synapse-associated protein 97, Disks large homolog 1
Background: Synapse-Associated Protein 97 (SAP97/human homologue of Drosophila discs-large tumor supressor or HDLG) is one of a family of plasma membrane-associated proteins found in synaptic junctions. This is a novel, presynaptic membrane protein homologous to SAP90 and the Drosophila discs-large tumor supressor protein. SAP97 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP97 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and Shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between SAP97 and other proteins. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif (2). Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP97 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP97 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
Gene Name: Dlg1
- 基因ID
- 25252
- NCBI登录号
- NP_036920
- UniProt
- Q62696
- 途径
- Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response
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