DLG1 抗体 (N-Term)
Quick Overview for DLG1 抗体 (N-Term) (ABIN6658116)
抗原
See all DLG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- N-Term
-
Supplier Product No.
- 200-301-g39
-
Supplier
- Rockland
-
原理
- SAP97 Antibody
-
交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with SAP97 from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.
-
纯化方法
- Anti-SAP97 Antibody was purified by Protein G chromatography.
-
过滤
- Sterile filtered
-
免疫原
- SAP97 Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an n-terminal region of rat SAP97.
-
亚型
- IgG1
-
-
anti-Discs, Large Homolog 1 (DLG1) (AA 662-773) antibody
DLG1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-90) antibodyDLG1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1D8 unconjugated
anti-Discs, Large Homolog 1 (DLG1) (Internal Region) antibodyDLG1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-190) antibodyDLG1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 643-673) antibodyDLG1 适用: 人 WB 宿主: 兔 Polyclonal RB53955 unconjugated
anti-Discs, Large Homolog 1 (DLG1) (Internal Region) antibodyVerified DLG1 适用: 人 ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-90) antibodyDLG1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D8 unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-90) antibodyDLG1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B12 unconjugated
anti-Discs, Large Homolog 1 (DLG1) (AA 1-165) antibodyDLG1 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Western_Blot_Dilution: 1:1000
-
说明
-
Anti-SAP97 Antibody is tested for use in WB, IHC and IP. Expect a band approximately ~130kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- DLG1 (Discs, Large Homolog 1 (DLG1))
-
别名
- SAP97
-
背景
-
Synonyms: Dlgh1, SAP97, Dlg1, Synapse-associated protein 97, Disks large homolog 1
Background: Synapse-Associated Protein 97 (SAP97/human homologue of Drosophila discs-large tumor supressor or HDLG) is one of a family of plasma membrane-associated proteins found in synaptic junctions. This is a novel, presynaptic membrane protein homologous to SAP90 and the Drosophila discs-large tumor supressor protein. SAP97 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP97 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and Shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between SAP97 and other proteins. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif (2). Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP97 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP97 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
Gene Name: Dlg1
-
基因ID
- 25252
-
NCBI登录号
- NP_036920
-
UniProt
- Q62696
-
途径
- Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response
抗原
-
