P4HB 抗体
Quick Overview for P4HB 抗体 (ABIN6658053)
抗原
See all P4HB 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-g29
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Supplier
- Rockland
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原理
- PDI Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with PDI from Human, Mouse, Rat, Dog, Hamster, Monkey, Guinea pig, Bovine, Sheep, Pig, and Xenopus based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-PDI Antibody was prepared from monospecific antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
- PDI Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against rat PDI synthetic peptide conjugated to KLH.
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亚型
- IgG
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anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 1-508) antibody
P4HB 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) antibodyP4HB 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) antibodyP4HB 适用: 人 WB, IF, ICC, IHC (p), IEM 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (N-Term) antibodyP4HB 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 329-508) antibodyP4HB 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 1-508) antibodyP4HB 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 409-509) antibodyP4HB 适用: 大鼠 WB, IHC, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (C-Term) antibodyP4HB 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, Pig, 兔, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 499-509) antibodyP4HB 适用: 人, 小鼠, 大鼠, Hamster, Cow, 犬, 猴, 豚鼠, Pig, 绵羊, 兔, 非洲爪蟾, 小鸡 WB, IHC, ICC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 409-509) antibody (HRP)P4HB 适用: 大鼠 WB, IHC, IP, IF, ICC 宿主: 兔 Polyclonal HRP
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Western_Blot_Dilution: 1:4000
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说明
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Anti-PDI Antibody is tested for use in WB, IP, IF microscopy and IHC. Expect a band approximately ~58kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
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别名
- PDI
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背景
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Synonyms: PDA2, PDI, PDIA2, PDIP, pancreatic protein disulfide isomerase, Protein disulfide-isomerase, PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta
Background: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins. Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins. PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase. PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol. PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus. It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
Gene Name: P4hb
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基因ID
- 287164
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NCBI登录号
- NP_001099245
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UniProt
- P04785
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途径
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
抗原
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