NEFM 抗体 (C-Term)
Quick Overview for NEFM 抗体 (C-Term) (ABIN6658052)
抗原
See all NEFM 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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Supplier Product No.
- 912-301-d84
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Supplier
- Rockland
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原理
- Neurofilament M Antibody
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交叉反应 (详细)
- Anti-NF-M antibody is directed against NF-M protein.
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纯化方法
- The antibody is protein G purified from concentrated clarified cell culture supernate.
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免疫原
- Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.
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亚型
- IgG1
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应用备注
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IF_Microscopy_Dilution: 1:100
Western_Blot_Dilution: 1:1000
Other: User Optimized
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说明
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Anti-NFM antibody is tested for use in Western Blot, IHC, and IF. Expect a band of approximately 145 kDa in size corresponding to the NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- NEFM (Neurofilament Triplet M Protein (NEFM))
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别名
- Neurofilament M
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背景
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Synonyms: mouse anti-Neurofilament M Antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
Background: NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-NF-M antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.
Gene Name: NEFM
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基因ID
- 24588
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UniProt
- P12839
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途径
- Brown Fat Cell Differentiation
抗原
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