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NEFM 抗体 (C-Term)

The 小鼠 单克隆 anti-NEFM antibody (Clone 3H11) (ABIN6658052) specifically detects NEFM in WB, IHC 和 FM. The antibody is reactive with 大鼠 samples.
Rockland
产品编号 ABIN6658052
Supplier Product No.: 912-301-d84
发货至: 中国
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Quick Overview for NEFM 抗体 (C-Term) (ABIN6658052)

抗原

See all NEFM 抗体
NEFM (Neurofilament Triplet M Protein (NEFM))

适用

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大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This NEFM antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

克隆位点

3H11
  • 抗原表位

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    C-Term

    Supplier Product No.

    912-301-d84

    Supplier

    Rockland

    原理

    Neurofilament M Antibody

    交叉反应 (详细)

    Anti-NF-M antibody is directed against NF-M protein.

    纯化方法

    The antibody is protein G purified from concentrated clarified cell culture supernate.

    免疫原

    Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.

    亚型

    IgG1
  • 应用备注

    IF_Microscopy_Dilution: 1:100

    Western_Blot_Dilution: 1:1000

    Other: User Optimized

    说明

    Anti-NFM antibody is tested for use in Western Blot, IHC, and IF. Expect a band of approximately 145 kDa in size corresponding to the NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    NEFM (Neurofilament Triplet M Protein (NEFM))

    别名

    Neurofilament M

    背景

    Synonyms: mouse anti-Neurofilament M Antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

    Background: NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-NF-M antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

    Gene Name: NEFM

    基因ID

    24588

    UniProt

    P12839

    途径

    Brown Fat Cell Differentiation
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