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NEFM 抗体 (C-Term)

Name: NEFM 抗体 (C-Term)
SKU: ABIN6658051
Availability: OutOfStock

NEFM 适用: 大鼠 WB, FM 宿主: 小鸡 Polyclonal unconjugated

Western blot of Anti-Neurofilament M (Chicken) Antibody - 212-901-D84 Western Blot of Chicken anti-Neurofilament M antibody. (NEFM 抗体 (C-Term))

1 image

(2 references)

产品编号 ABIN6658051

发货至: 中国

Datasheet as PDF

抗原 See all NEFM 抗体

NEFM (Neurofilament Triplet M Protein (NEFM))
抗原表位
17

16

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
58

47

39

8

6

6

6

6

5

5

4

4

3

2

2

1

1
大鼠
宿主
39

23

11

1
小鸡
克隆类型
43

31
多克隆
标记
52

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This NEFM antibody is un-conjugated
应用范围
66

24

21

17

13

13

13

12

11

11

4

2

1

1

1
Western Blotting (WB), Fluorescence Microscopy (FM)

交叉反应

小鸡, 牛, 人, 小鼠, 大鼠

交叉反应（详细）

Cross reactivity with NF-M from other species has not been determined.

纯化方法

Anti-NF-M antibody is directed against NF-M protein. The antibody is a total IgY fraction. Expect reactivity with the following species based on sequence homology: chicken, human, mouse.

免疫原

Immunogen: Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.
Immunogen Type: Recombinant Protein

亚型

IgG

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NEFM 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Neurofilament Triplet M Protein (NEFM) antibody
NEFM 适用: 人, 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated

应用备注

Application Note: Anti- Neurofilament M antibody is suitable for use in Western Blotting and IF. Specific . Expect a band of approximately 145 kDa in size corresponding to NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:5000
IF Microscopy Dilution: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Martineau, Di Polo, Vande Velde, Robitaille: "Dynamic neuromuscular remodeling precedes motor-unit loss in a mouse model of ALS." in: eLife, Vol. 7, (2019) (PubMed).

Darabid, Arbour, Robitaille: "Glial cells decipher synaptic competition at the mammalian neuromuscular junction." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 4, pp. 1297-313, (2013) (PubMed).
抗原

NEFM (Neurofilament Triplet M Protein (NEFM))

别名

Neurofilament M (NEFM 产品)

别名

NEF3 antibody, NF-M antibody, NFM antibody, Nef3 antibody, Nfm antibody, NF160 antibody, NF165 antibody, nef-m antibody, nef3 antibody, nefm antibody, nf-m antibody, zgc:112359 antibody, nefm-a antibody, nefm-b antibody, neurofilament medium antibody, neurofilament triplet M protein antibody, neurofilament, medium polypeptide antibody, neurofilament medium polypeptide antibody, neurofilament medium L homeolog antibody, neurofilament, medium polypeptide a antibody, neurofilament medium S homeolog antibody, NEFM antibody, Nefm antibody, TVAG_582010 antibody, TVAG_379630 antibody, nefm.L antibody, nefma antibody, nefm.S antibody

背景

Synonyms: chicken anti-Neurofilament M antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
Background: NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Neurofilament M Antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.
Gene Name: NEFM

基因ID

24588

UniProt

P12839

途径

Brown Fat Cell Differentiation