NEFM 抗体 (C-Term)
(6 references)
Quick Overview for NEFM 抗体 (C-Term) (ABIN6658051)
抗原
See all NEFM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 212-901-d84
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Supplier
- Rockland
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原理
- Neurofilament M Antibody
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交叉反应 （详细）
- Anti-NF-M antibody is directed against NF-M protein.
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纯化方法
- The antibody is a total IgY fraction.
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免疫原
- Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.
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亚型
- IgG
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anti-Neurofilament Triplet M Protein (NEFM) (AA 779-916) antibody
NEFM 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1B6B12 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyNEFM 适用: 哺乳动物 WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal NF-09 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 381-443) antibodyNEFM 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 101-200) antibodyNEFM 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 105-287) antibodyNEFM 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 200-300) antibodyNEFM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (C-Term) antibodyNEFM 适用: 大鼠 WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (C-Term) antibodyNEFM 适用: 大鼠 WB, IF 宿主: 小鼠 Monoclonal 3H11 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (Internal Region) antibodyVerified NEFM 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyNEFM 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
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应用备注
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IF_Microscopy_Dilution: 1:1000
Western_Blot_Dilution: 1:5000
Other: User Optimized
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说明
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Suggested Applications: IHC
Anti- Neurofilament M antibody is tested for use in Western Blotting and IF. Specific . Expect a band of approximately 145 kDa in size corresponding to NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Kir4.1 is specifically expressed and active in non-myelinating Schwann cells." in: Glia, Vol. 71, Issue 4, pp. 926-944, (2023) (PubMed).
: "Functional adaptation of glial cells at neuromuscular junctions in response to injury." in: Glia, Vol. 70, Issue 9, pp. 1605-1629, (2022) (PubMed).
: "Properties of Glial Cell at the Neuromuscular Junction Are Incompatible with Synaptic Repair in the SOD1G37R ALS Mouse Model." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 40, Issue 40, pp. 7759-7777, (2021) (PubMed).
: "Purinergic-Dependent Glial Regulation of Synaptic Plasticity of Competing Terminals and Synapse Elimination at the Neuromuscular Junction." in: Cell reports, Vol. 25, Issue 8, pp. 2070-2082.e6, (2019) (PubMed).
: "Dynamic neuromuscular remodeling precedes motor-unit loss in a mouse model of ALS." in: eLife, Vol. 7, (2019) (PubMed).
: "Glial cells decipher synaptic competition at the mammalian neuromuscular junction." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 4, pp. 1297-313, (2013) (PubMed).
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: "Kir4.1 is specifically expressed and active in non-myelinating Schwann cells." in: Glia, Vol. 71, Issue 4, pp. 926-944, (2023) (PubMed).
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- NEFM (Neurofilament Triplet M Protein (NEFM))
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别名
- Neurofilament M
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背景
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Synonyms: chicken anti-Neurofilament M antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
Background: NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Neurofilament M Antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.
Gene Name: NEFM
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基因ID
- 24588
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UniProt
- P12839
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途径
- Brown Fat Cell Differentiation
抗原
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