TNNI3 抗体 (pSer23, pSer24)
Quick Overview for TNNI3 抗体 (pSer23, pSer24) (ABIN6658033)
抗原
See all TNNI3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer23, pSer24
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Supplier Product No.
- 600-401-x32
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Supplier
- Rockland
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原理
- Troponin I (cardiac) Phospho S23/24 Antibody
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交叉反应 （详细）
- Anti-Phospho-Ser23/24 Troponin (cardiac) Antibody is directed against Troponin protein phosphorylated at S23 and S24.
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纯化方法
- The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Anti-Phospho-Ser23/24 Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser23/24 of mouse cardiac troponin I conjugated to KLH.
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亚型
- IgG
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anti-Cardiac Troponin I (TNNI3) (AA 102-210) antibody
TNNI3 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 1E7 unconjugated
anti-Cardiac Troponin I (TNNI3) (AA 1-211) antibodyTNNI3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin I (TNNI3) (AA 2-211) antibodyTNNI3 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin I (TNNI3) antibodyTNNI3 适用: 人, 犬, 猴 WB, IF 宿主: 小鼠 Monoclonal 4E5 unconjugated
anti-Cardiac Troponin I (TNNI3) (AA 1-211) antibodyTNNI3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin I (TNNI3) (AA 117-127) antibodyVerified TNNI3 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Cardiac Troponin I (TNNI3) (AA 2-210) antibodyTNNI3 适用: 人 ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin I (TNNI3) (pSer44) antibodyTNNI3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin I (TNNI3) (AA 1-203) antibodyTNNI3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiac Troponin I (TNNI3) (N-Term) antibodyTNNI3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000
Western_Blot_Dilution: 1:1000
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说明
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Anti-Phospho-Ser23/24 Troponin (cardiac) Antibody has been tested in Western Blots and is specific for the ~25 kDa cardiac troponin I protein phosphorylated at Ser 23/24. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
Preservative: None -
储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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有效期
- 12 months
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- TNNI3 (Cardiac Troponin I (TNNI3))
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别名
- Troponin I (cardiac)
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背景
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Synonyms: Troponin I cardiac muscle, Cardiac troponin I
Background: Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place, and when cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 it causes regulation of Ca2+- stimulated ATPase. Evidence suggests that phosphorylation of both serines 23 and 24 is required for the reduction in Ca2+-sensitivity and beneficial for relaxation of the heart. Ser23/24 phosphorylation is important for enhanced relaxation in response to prolonged activation of protein kinase C by endothelin in intact myocytes, while Thr144 plays an important role in the acute acceleration of relaxation. Troponin I pS23/24 Antibody is ideal for researchers interested in cancer research.
Gene Name: TNNI3
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基因ID
- 21954
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NCBI登录号
- NP_033432
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UniProt
- P48787
抗原
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