GADD45G 抗体 (AA 142-159)
Quick Overview for GADD45G 抗体 (AA 142-159) (ABIN6658028)
抗原
See all GADD45G 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 142-159
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Supplier Product No.
- 100-401-863
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Supplier
- Rockland
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原理
- GADD45 gamma Antibody
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交叉反应 (详细)
- Reactivity occurs against mouse PI3K p110d subunit.
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纯化方法
- This antibody was prepared from whole rabbit antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
- This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 142-159 of Mouse GADD45 gamma. Swiss Prot accession number Q9Z111. This sequence represents the C-terminal end of the protein. Mouse and rat GADD45 g show 100% sequence homology. A single amino acid change is found in the human sequence (see below).
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亚型
- IgG
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应用备注
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Immunoprecipitation_Dilution: Use 10 μL
ELISA_Dilution: 1:10,000 - 1:50,000
Western_Blot_Dilution: 1:100 - 1:500
Other: User Optimized
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说明
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This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Reactivity in other immunoassays is unknown. This product was assayed by western blotting after immunoprecipitation of mouse GADD45 gamma from a lysate containing over expressed protein. A 1:100 to 1:500 dilution shows reactivity with a band at 19.2kDa corresponding to mouse GADD45 gamma. Use 10 μL for immunoprecipitation.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))
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别名
- GADD45 gamma
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背景
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Synonyms: rabbit anti-GADD45 gamma Antibody, Cytokine responsive protein CR6 antibody, DDIT2 antibody, GADD45G antibody antibody, Growth arrest and DNA damage inducible gamma antibody, GRP17 antibody
Background: GADD45 gamma (Growth arrest and DNA-damage-inducible protein also known as cytokine responsive protein CR6) is derived from a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45 gamma is highly expressed in placenta.
Gene Name: GADD45 gamma
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基因ID
- 23882
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NCBI登录号
- NP_035947
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UniProt
- Q9Z111
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途径
- Cell Division Cycle
抗原
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