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SIX3 抗体 (Internal Region)

This anti-SIX3 antibody is a 豚鼠 多克隆 antibody detecting SIX3 in WB, ELISA, IHC, IF, FM 和 DB. Suitable for 小鼠. This Primary Antibody has been cited in 5+ publications.
Rockland
产品编号 ABIN6657910
Supplier Product No.: 200-201-a26
发货至: 中国

Quick Overview for SIX3 抗体 (Internal Region) (ABIN6657910)

抗原

See all SIX3 抗体
SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

适用

  • 37
  • 13
  • 9
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  • 3
  • 2
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  • 2
  • 1
小鼠

宿主

  • 37
  • 3
  • 1
  • 1
豚鼠

克隆类型

  • 40
  • 2
多克隆

标记

  • 23
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This SIX3 antibody is un-conjugated

应用范围

  • 41
  • 18
  • 13
  • 13
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  • 3
  • 3
  • 3
  • 2
  • 1
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Dot Blot (DB)
  • 抗原表位

    • 15
    • 8
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
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    Internal Region

    Supplier Product No.

    200-201-a26

    Supplier

    Rockland

    原理

    SIX3 Antibody

    交叉反应 (详细)

    This antibody reacts with mouse Six3.

    纯化方法

    This product was purified by Protein A chromatography from monospecific antiserum.

    过滤

    Sterile filtered

    免疫原

    This Protein A purified antibody was prepared from whole guinea pig serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Six3 protein.

    亚型

    IgG
  • 应用备注

    ELISA_Dilution: 1:5,000 - 1:25,000

    Immunohistochemistry_Dilution: 1:250 - 1:500

    IF_Microscopy_Dilution: 1:250 - 1:500

    Western_Blot_Dilution: 1:500 - 1:2,000

    Other: User Optimized

    说明

    Suggested Applications: Multiplex
    This Protein A purified antibody has been tested for use in Immunohistochemistry, immunofluorescence microscopy, dot blot, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 37 kDa in size corresponding to Six3 by western blotting in the appropriate cell lysate or extract.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Lipiec, Bem, Koziński, Chakraborty, Urban-Ciećko, Zajkowski, Dąbrowski, Szewczyk, Toval, Ferran, Nagalski, Wiśniewska: "TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus." in: Development (Cambridge, England), Vol. 147, Issue 16, (2021) (PubMed).

    Grall, Gourain, Naïr, Martin, Birling, Freund, Duluc: "Severe head dysgenesis resulting from imbalance between anterior and posterior ontogenetic programs." in: Cell death & disease, Vol. 10, Issue 11, pp. 812, (2020) (PubMed).

    OBrien, Guo, Bahrami-Samani, Park, Hasso, Lee, Fang, Kim, Guo, Hong, Peterson, Lozanoff, Raviram, Ren, Fogelgren, Smith, Valouev, McMahon: "Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies." in: PLoS genetics, Vol. 14, Issue 1, pp. e1007181, (2018) (PubMed).

    Madrigal, Moreno-Bravo, Martínez-López, Martínez, Puelles: "Mesencephalic origin of the rostral Substantia nigra pars reticulata." in: Brain structure & function, Vol. 221, Issue 3, pp. 1403-12, (2016) (PubMed).

    Moldrich, Gobius, Pollak, Zhang, Ren, Brown, Mori, De Juan Romero, Britanova, Tarabykin, Richards: "Molecular regulation of the developing commissural plate." in: The Journal of comparative neurology, Vol. 518, Issue 18, pp. 3645-61, (2010) (PubMed).

  • 抗原

    SIX3 (Sine Oculis-Related Homeobox 3 (SIX3))

    别名

    SIX3

    背景

    Synonyms: guinea pig anti-SIX 3 antibody, Six3, Sine oculis homeobox homolog 3

    Background: Six3 (also known as sine oculis homeobox homolog 3) is involved in the development of the visual system and forebrain.  Six3 is a nuclear protein that is reported to exist in two forms by alternative splicing of the gene product.  Six3 is first expressed at E6.5 of mouse embryonic development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, it is in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke's pouch. In later stages, Six3 is present in hypothalamus, eyes and pituitary.

    Gene Name: SIX3

    基因ID

    20473, 59939908

    UniProt

    Q62233

    途径

    Protein targeting to Nucleus
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