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TCRb 抗体 (FITC)

This anti-TCRb antibody (ABIN6657906) is a Hamster Monoclonal antibody detecting TCRb in FACS, IP, FM. Suitable for Mouse.
Rockland
产品编号 ABIN6657906
Supplier Product No.: 200-b02-n92
发货至: 中国

Quick Overview for TCRb 抗体 (FITC) (ABIN6657906)

抗原

See all TCRb 抗体
TCRb (T Cell Receptor beta Chain (TCRb))

适用

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小鼠

宿主

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仓鼠

克隆类型

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单克隆

标记

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This TCRb antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

克隆位点

H57-597
  • Supplier Product No.

    200-b02-n92

    Supplier

    Rockland

    原理

    TCRbeta Fluorescein Antibody

    交叉反应 (详细)

    Cross reactivity with TCRbeta from other sources has not been tested.

    纯化方法

    Fluorescein conjugated TCRbeta Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse TCRbeta.

    过滤

    Sterile filtered

    免疫原

    Anti-TCRbeta Antibody (Monoclonal) was produced by repeated immunizations with TCRbeta antigen.

    亚型

    IgG

    Labeling Ratio

    2-8
  • 应用备注

    Immunoprecipitation_Dilution: User Optimized

    Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

    IF_Microscopy_Dilution: User Optimized

    Other: Sufficient to run approximately 500 tests

    说明

    Anti-TCR beta is tested for FLOW and useful for Immunofluorescence and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.1 % Gelatin

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

    有效期

    6 months
  • 抗原

    TCRb (T Cell Receptor beta Chain (TCRb))

    别名

    Tcrb

    背景

    Synonyms: Tib, TCRbeta, T cell receptor beta chain, Tcrb

    Background: The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the gamma-delta TCR.

    Gene Name: Tcrb

    基因ID

    21577
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