XIRP1 抗体 (pSer295)
Quick Overview for XIRP1 抗体 (pSer295) (ABIN6657875)
抗原
See all XIRP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer295
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Supplier Product No.
- 600-401-x37
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Supplier
- Rockland
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原理
- Xin Actin-binding Repeat-containing Protein 1 Phospho S295 (XIRP1) Antibody
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交叉反应 （详细）
- Anti-Phospho-Ser295 Xin Actin-Binding Repeat Containing Protein 1 Antibody is directed against Xin Actin-Binding Repeat Containing Protein 1 phosphorylated at S295.
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纯化方法
- The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Anti-Phospho-Ser295 Xin Actin-Binding Repeat Containing Protein 1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of mouse Xin Actin-binding Repeat-containing Protein 1 conjugated to KLH.
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亚型
- IgG
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anti-Xin Actin-Binding Repeat Containing 1 (XIRP1) antibody
XIRP1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Xin Actin-Binding Repeat Containing 1 (XIRP1) (AA 1338-1367), (C-Term) antibodyXIRP1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33080 unconjugated
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应用备注
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ELISA_Dilution: 1:10,000
Western_Blot_Dilution: 1:1000
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说明
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Anti-XIRP1pS295 Antibody has been tested by Western Blots and is specific for the ~150 kDa and ~250 kDa splice variants of XIRP1 phosphorylated at Ser295. Immunolabeling of the XIRP1 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
Preservative: None -
储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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有效期
- 12 months
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- XIRP1 (Xin Actin-Binding Repeat Containing 1 (XIRP1))
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别名
- Xin Actin-binding Repeat-containing Protein 1 (XIRP1)
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背景
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Synonyms: XIRP1, Cardiomyopathy-associated protein 1
Background: XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons. The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers. Loss of mXinα (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy. Phosphorylation of Ser295 is thought to play a key role in XIRP signaling. Xin Actin-Binding Repeat Containing Protein 1 pS295 Antibody is ideal for researchers interested in cell cycle research.
Gene Name: XIRP1
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基因ID
- 22437
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UniProt
- O70373
抗原
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