NK-1.1/CD161c 抗体 (FITC)
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北京 101111
Quick Overview for NK-1.1/CD161c 抗体 (FITC) (ABIN6657870)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-302-n93
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Supplier
- Rockland
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原理
- NK1.1 Fluorescein Antibody
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纯化方法
- Fluorescein conjugated NK1.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse NK1.
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过滤
- Sterile filtered
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免疫原
- Anti-NK1.1 Antibody (Monoclonal) was produced by repeated immunizations with NK1.1 antigen.
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亚型
- IgG2a kappa
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anti-NK-1.1 (AA 101-200) antibody
适用: 小鼠, 人 FACS, WB, IF (cc), IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-NK-1.1 antibody (PerCP-Cy5.5)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal PK136 PerCP-Cy5.5
anti-NK-1.1 antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal PK136 PE-Cy7
anti-NK-1.1 antibody适用: 小鼠 FACS, IP, CyTox 宿主: 小鼠 Monoclonal PK136 unconjugated
anti-NK-1.1 antibody适用: 小鼠 FACS 宿主: 小鼠 Monoclonal PK136 unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)
IF_Microscopy_Dilution: User Optimized
Other: Sufficient to run approximately 500 tests
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说明
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Anti-NK1.1 has been tested by Flow Cytometry and is useful for Immunohistochemistry, Immunoprecipitation, Immunofluorescence using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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有效期
- 6 months
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- NK-1.1/CD161c (NK-1.1)
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别名
- NK1.1
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背景
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Synonyms: Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c
Background: The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1. The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1.
Gene Name: Klrb1c
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基因ID
- 17059
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NCBI登录号
- NP_001153376
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UniProt
- P27814
抗原
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