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LGI1 抗体 (AA 37-113) (FITC)

This anti-LGI1 antibody is a 小鼠 单克隆 antibody detecting LGI1 in WB, IHC 和 ELISA. Suitable for 小鼠.
Rockland
产品编号 ABIN6657730
Supplier Product No.: 200-302-w73
发货至: 中国

Quick Overview for LGI1 抗体 (AA 37-113) (FITC) (ABIN6657730)

抗原

See all LGI1 抗体
LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

适用

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  • 1
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  • 1
  • 1
小鼠

宿主

  • 29
  • 22
小鼠

克隆类型

  • 29
  • 22
单克隆

标记

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  • 3
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  • 2
  • 2
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  • 1
  • 1
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This LGI1 antibody is conjugated to FITC

应用范围

  • 50
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

克隆位点

S283-7
  • 抗原表位

    • 16
    • 15
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
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    AA 37-113

    Supplier Product No.

    200-302-w73

    Supplier

    Rockland

    原理

    Lgi1 Antibody Fluorescein

    交叉反应 (详细)

    BLAST analysis suggests that it is 100 % identical to rat, 98 % identical to human and ~50 % identical to LGI2, LGI3, and LGI4.

    纯化方法

    Anti-LGI1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

    过滤

    Sterile filtered

    免疫原

    Anti-LGI1 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1.

    亚型

    IgG2a
  • 应用备注

    ELISA_Dilution: 1:10,000

    Immunohistochemistry_Dilution: User Optimized

    Western_Blot_Dilution: 1:1000

    说明

    Anti-LGI1 FITC Conjugated Antibody is suitable for Western Blots, Immunohistochemistry and Immunocytochemistry. Expect a band approximately ~60kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.1 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

    别名

    LGI1

    背景

    Synonyms: ADLTE, ADPAEF, ADPEAF, Epitempin 1, EPT, ETL1, IB1099, leucine rich glioma inactivated 1, OTTHUMP00000020121, OTTHUMP00000020134

    Background: The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist, this LGI1 antibody will recognize only the longer form. Anti-LGI1 is ideal for research in Neuroscience and Cancer.

    Gene Name: LGI1

    基因ID

    56839

    UniProt

    Q9JIA1
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