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MYOD1 抗体 (Internal Region)

The 小鼠 单克隆 anti-MYOD1 antibody (Clone 5-8A) (ABIN6657593) specifically detects MYOD1 in WB, IHC, IF, FACS 和 IP. The antibody is reactive with 小鼠 samples.
Rockland
产品编号 ABIN6657593
Supplier Product No.: 200-301-h94
发货至: 中国
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Quick Overview for MYOD1 抗体 (Internal Region) (ABIN6657593)

抗原

See all MYOD1 抗体
MYOD1 (Myogenic Differentiation 1 (MYOD1))

适用

  • 162
  • 76
  • 70
  • 31
  • 2
  • 1
小鼠

宿主

  • 104
  • 61
  • 1
小鼠

克隆类型

  • 100
  • 66
  • 1
单克隆

标记

  • 78
  • 11
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This MYOD1 antibody is un-conjugated

应用范围

  • 87
  • 49
  • 48
  • 43
  • 43
  • 22
  • 20
  • 18
  • 18
  • 14
  • 12
  • 9
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

克隆位点

5-8A
  • 抗原表位

    • 16
    • 13
    • 9
    • 9
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    Supplier Product No.

    200-301-h94

    Supplier

    Rockland

    原理

    MyoD Antibody

    交叉反应 (详细)

    A BLAST analysis was used to suggest cross-reactivity with Anti-MyoD from Human, Cat, Rat, and Mouse based on 100 % homology with the immunizing sequence.

    纯化方法

    Anti-MyoD Antibody was purified by Protein G chromatography.

    免疫原

    MyoD Antibody was produced in mice prepared by repeated immunizations with recombinant mouse MyoD protein at an internal amino acid sequence.

    亚型

    IgG1 kappa
  • 应用备注

    Immunoprecipitation_Dilution: 1 μg/mL

    Immunohistochemistry_Dilution: 5 μg/mL

    Western_Blot_Dilution: 1 μg/mL

    说明

    Anti-MyoD Antibody is tested for use in WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 45kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.02 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    MYOD1 (Myogenic Differentiation 1 (MYOD1))

    别名

    MyoD

    背景

    Synonyms: Myod, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3

    Background: Anti-MyoD Antibody detects MyoD. The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are: myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin). Anti-MyoD Antibody is ideal for investigators involved in cell cycle protein, cytokines and growth factors.

    Gene Name: Myod1

    基因ID

    17927

    NCBI登录号

    NP_034996

    UniProt

    P10085

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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