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DDT 抗体 (C-Term)

Cited in 1 publication. This anti-DDT antibody is a 兔 多克隆 antibody detecting DDT in WB 和 IHC. Suitable for 人.
Rockland
产品编号 ABIN6657521
Supplier Product No.: 600-401-r05
发货至: 中国
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Quick Overview for DDT 抗体 (C-Term) (ABIN6657521)

抗原

See all DDT 抗体
DDT (D-Dopachrome Tautomerase (DDT))

适用

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宿主

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克隆类型

  • 43
  • 6
多克隆

标记

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This DDT antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 抗原表位

    • 15
    • 8
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    C-Term

    Supplier Product No.

    600-401-r05

    Supplier

    Rockland

    原理

    DDT Antibody

    交叉反应 (详细)

    Anti-DDT antibody is directed against human DDT protein.

    纯化方法

    The product was affinity purified from monospecific antiserum by immunoaffinity purification.

    免疫原

    DDT affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human DDT.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry_Dilution: 1:100-1:500

    Western_Blot_Dilution: 0.5 μg/mL

    说明

    Suggested Applications: IP
    Anti-DDT is tested for Immunohistochemistry-P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~12.7 kDa corresponding to the appropriate cell lysate or extract.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 200μL

    缓冲液

    Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

    Stabilizer: 5 mg BSA

    Preservative: 0.05 mg Thimerosal

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Song, Liu, Habibie, van den Bor, Smit, Gosens, Wu, Brandsma, Cool, Haisma, Poelarends, Melgert: "D-dopachrome tautomerase contributes to lung epithelial repair via atypical chemokine receptor 3-dependent Akt signaling." in: EBioMedicine, Vol. 68, pp. 103412, (2021) (PubMed).

  • 抗原

    DDT (D-Dopachrome Tautomerase (DDT))

    别名

    DDT

    背景

    Synonyms: D-dopachrome tautomerase,Phenylpyruvate tautomerase II

    Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure. The genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10. This antibody is suitable for researchers interested in stem cell research.

    Gene Name: DDT

    基因ID

    1652

    NCBI登录号

    NP_001077861

    UniProt

    P30046
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