EEF1A2 抗体 (pSer358)
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- 抗原 See all EEF1A2 抗体
- EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))
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抗原表位
- pSer358
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EEF1A2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应
- 小鼠
- 纯化方法
- Anti-Phospho-Ser358 eEF1A2 Antibody is directed against human eEF1A2 protein phosphorylated at S358. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated eEF1A2. Minimal reactivity occurs against non-phosphorylated eEF1A2. Reactivity against eEF1A2 occurs from mouse sources. However, reactivity is also expected against chicken, human, non-human primate, rat, sheep, and Xenopus based on 100% sequence homology.
- 免疫原
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Immunogen: Anti-Phospho-Ser358 eEF1A2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2 conjugated to KLH.
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product EEF1A2 Primary Antibody
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anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) antibody
KO Validated EEF1A2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) (AA 364-462) antibodyEEF1A2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) (AA 46-72) antibodyEEF1A2 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB19607 unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) (AA 238-443) antibodyEEF1A2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) (Middle Region) antibodyEEF1A2 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) (AA 184-463) antibodyEEF1A2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) (Internal Region) antibodyEEF1A2 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) (Internal Region) antibodyVerified EEF1A2 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) (pSer358) antibodyEEF1A2 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2) antibodyEEF1A2 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-Phospho-Ser358 eEF1A2 Antibody is suitable for Western Blots and is specific for the ~50 kDa eEF1A2 protein phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10,000
Western Blot Dilution: 1:1000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
- 储存液
- Without preservative
- 储存条件
- RT,-20 °C
- 储存方法
- Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut
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- 抗原
- EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))
- 别名
- eEF1A2 (EEF1A2 产品)
- 别名
- EEF1AL antibody, EF-1-alpha-2 antibody, EF1A antibody, HS1 antibody, STN antibody, STNL antibody, EEF1A2 antibody, Eef1a antibody, S1 antibody, wasted antibody, wst antibody, Ps10 antibody, RATPS10 antibody, Stnl antibody, eef1al antibody, ef1a antibody, stnl antibody, EEF1A-2 antibody, zgc:92085 antibody, eukaryotic translation elongation factor 1 alpha 2 antibody, eukaryotic translation elongation factor 1 alpha 1 antibody, eukaryotic translation elongation factor 1 alpha 2 S homeolog antibody, putative elongation factor 1-alpha-like 3 antibody, EEF1A2 antibody, EEF1A1 antibody, Eef1a2 antibody, eef1a2.S antibody, eef1a2 antibody, LOC739210 antibody, EEF1 antibody, eef1 antibody
- 背景
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Synonyms: elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1
Background: Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle. Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors. Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites. Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome. eEF1A2 pS358 Antibody is ideal for researchers interested in cancer research.
Gene Name: EEF1A2
- 基因ID
- 1917
- NCBI登录号
- NP_001949
- UniProt
- Q05639
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