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Bassoon 抗体 (N-Term)

This anti-Bassoon antibody is a 兔 多克隆 antibody detecting Bassoon in IHC, WB, FM 和 MA. Suitable for 人.
Rockland
产品编号 ABIN6657297
Supplier Product No.: 600-401-e93
发货至: 中国

Quick Overview for Bassoon 抗体 (N-Term) (ABIN6657297)

抗原

See all Bassoon (BSN) 抗体
Bassoon (BSN)

适用

  • 23
  • 14
  • 13

宿主

  • 24
  • 6
  • 1

克隆类型

  • 24
  • 5
  • 1
多克隆

标记

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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Bassoon antibody is un-conjugated

应用范围

  • 22
  • 21
  • 4
  • 4
  • 3
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM), Multiplex Assay (MA)
  • 抗原表位

    • 13
    • 2
    • 1
    • 1
    • 1
    N-Term

    Supplier Product No.

    600-401-e93

    Supplier

    Rockland

    原理

    BASSOON Antibody

    交叉反应 (详细)

    A BLAST analysis was used to suggest cross-reactivity with Bassoon from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

    纯化方法

    Anti-Bassoon Antibody was purified by affinity chromatography.

    过滤

    Sterile filtered

    免疫原

    Bassoon Antibody was produced from whole rabbit serum prepared by repeated immunizations with a N-terminal his-tagged fusion protein.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry_Dilution: User Optimized

    IF_Microscopy_Dilution: 1:400

    Western_Blot_Dilution: 1:1000

    说明

    Anti-Bassoon Antibody is tested for use in WB and IHC. Expect a band approximately ~420kDa on specific lysates corresponding to the molecular mass of Bassoon. Multiple isoforms can be detected. Specific conditions for reactivity should be optimized by the end user. Product provided in PBS pH 7.4.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: See application note.

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    Bassoon (BSN)

    别名

    Bassoon

    背景

    Synonyms: BSN, ZNF231, Neuronal double zine finger protein, protein bassoon

    Background: Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors.

    Gene Name: BSN

    基因ID

    8927

    NCBI登录号

    NP_003449

    UniProt

    Q9UPA5
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