FTO 抗体 (N-Term)
Quick Overview for FTO 抗体 (N-Term) (ABIN6657007)
抗原
See all FTO 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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Supplier Product No.
- 200-301-d41
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Supplier
- Rockland
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原理
- FTO Antibody
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交叉反应 (详细)
- Anti-FTO antibody is directed against human Fat mass and Obesity Related Protein.
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纯化方法
- Anti-FTO is a Protein G purified antibody from cell culture supernatant.
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免疫原
- Anti-FTO Monoclonal Antibody was produced in mouse by repeated immunizations with FTO- Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
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亚型
- IgG
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应用备注
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IF_Microscopy_Dilution: 1:100
Western_Blot_Dilution: 1:1000
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说明
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Anti-FTO (Mouse) Antibody has been tested in Western Blotting and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 58 kDa in size corresponding to FTO proteins in Western blots of rat testes lysate. This antibody has also been used for immunocytochemistry with neuronal progenitor cells.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- FTO (Fat Mass and Obesity-Associated (FTO))
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别名
- FTO
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背景
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Synonyms: Alpha-ketoglutarate-dependent dioxygenase FTO, KIAA1752
Background: FTO Antibody detects human FTO which is the protein encoded by FTO gene that is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake. Deletion analysis of FTO gene in mice showed that FTO is functionally involved in the control of both energy intake and energy expenditure. Anti-FTO Antibody is ideal for investigators involved in Neuroscience and Cell Metabolism.
Gene Name: FTO
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基因ID
- 79068
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UniProt
- Q9C0B1
抗原
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