DNAJB1 抗体
Quick Overview for DNAJB1 抗体 (ABIN6656695)
抗原
See all DNAJB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-f56
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Supplier
- Rockland
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原理
- HSP40 (HDJ1) Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Hsp40 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Hsp40 Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- Hsp40 Antibody was produced in mice by repeated immunizations raised against recombinant Protein Hsp40 (Hdj1).
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亚型
- IgG1
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anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (C-Term) antibody
DNAJB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 1-340) antibodyDNAJB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 2-340) antibodyDNAJB1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 1-340) antibodyDNAJB1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal 3B9-E6 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB1770 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) (AA 74-123) antibodyDNAJB1 适用: 人, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibodyDNAJB1 适用: Saccharomyces cerevisiae WB, ELISA, IP 宿主: 小鼠 Monoclonal 2A7-H6 unconjugated
anti-DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody (HRP)DNAJB1 适用: 人 WB, ELISA, IHC, IP, IF, ICC 宿主: 小鼠 Monoclonal 3B9-E6 HRP
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应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: 1:200
Immunohistochemistry_Dilution: User Optimized
IF_Microscopy_Dilution: 10 μg/mL
Western_Blot_Dilution: 1:2000
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说明
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Anti-Hsp40 Antibody is tested for use in WB, IP, IHC, IF, and ELISA. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
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别名
- Hsp40 (Hdj1)
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背景
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Synonyms: DNAJ1, NDAJB1, HDJ1, Hsp40, HSPF1, DnaJ homolog subfamily B member 1, Dna J protein homolog 1, Heat shock 40 kDa protein 1, HSP40, heat shock protein 40, Human DnaJ protein 1, hDj-1
Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1. Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding.
Gene Name: DNAJB1
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基因ID
- 3337
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NCBI登录号
- NP_006136
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UniProt
- P25685
抗原
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