Rab4 抗体 (C-Term)
Quick Overview for Rab4 抗体 (C-Term) (ABIN6656358)
抗原
See all Rab4 (RAB4A) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 100-401-g35
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Supplier
- Rockland
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原理
- RAB4 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Rab4 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Rab4 Antibody was prepared from monospecific antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
- Rab4 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against a C-terminal region synthetic peptide from human Rab4.
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亚型
- IgG
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anti-RAB4A, Member RAS Oncogene Family (RAB4A) antibody
RAB4A 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 4E11 unconjugated
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibodyRAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (AA 1-218) antibodyRAB4A 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1C10 unconjugated
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (HRP)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (FITC)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (Biotin)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (PE)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal PE
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (PerCP)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal PerCP
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (APC)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal APC
anti-RAB4A, Member RAS Oncogene Family (RAB4A) (C-Term) antibody (Atto 488)RAB4A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Atto 488
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应用备注
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Immunohistochemistry_Dilution: 1:100
Western_Blot_Dilution: 1:1000-2000
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说明
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Anti-Rab4 Antibody is tested by WB, IHC, and IF microscopy. Expect a band approximately ~26kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))
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别名
- Rab4
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背景
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Synonyms: Oncogene Rab4, Rab4A, Ras related protein Rab-4A
Background: Rab4 is a 25 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment. Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COOH-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. Each Rab shows a characteristic sub-cellular distribution. In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.
Gene Name: RAB4A
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基因ID
- 5867
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NCBI登录号
- NP_004569
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UniProt
- P20338
抗原
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