HSPB8 抗体
Quick Overview for HSPB8 抗体 (ABIN6656215)
抗原
See all HSPB8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-f53
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Supplier
- Rockland
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原理
- HSP22 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Hsp22 from human, mouse, and rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Hsp22 Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- Hsp22 Antibody was produced in mice by repeated immunizations raised against a his-tagged human recombinant hsp22.
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亚型
- IgG1 kappa
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anti-Heat Shock 22kDa Protein 8 (HSPB8) (AA 1-196) antibody
HSPB8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibodyHSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 22kDa Protein 8 (HSPB8) (AA 97-196) antibodyHSPB8 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 5B12 unconjugated
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (HRP)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (FITC)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (Biotin)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (PE)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (APC)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal APC
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (PerCP)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PerCP
anti-Heat Shock 22kDa Protein 8 (HSPB8) antibody (Atto 488)HSPB8 适用: 人 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Atto 488
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应用备注
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ELISA_Dilution: 1:200
Immunohistochemistry_Dilution: User Optimized
Western_Blot_Dilution: 1:2000
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说明
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Anti-Hsp22 Antibody is tested for use in WB, IHC and IF. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))
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别名
- Hsp22
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背景
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Synonyms: Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, Heat shock 22 kDa protein 8, HMN2, HSB8, HspB8, Heat shock protein beta-8, E2IG1, HSP22, E2-induced gene 1 protein, Protein kinase H11
Background: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27. HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).
Gene Name: HSPB8
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基因ID
- 26353
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NCBI登录号
- NP_055180
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UniProt
- Q9UJY1
抗原
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