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GFAP 抗体 (AA 411-422) (Biotin)

The 小鼠 单克隆 anti-GFAP antibody is suitable to detect GFAP in samples from 人. It has been validated for WB, IHC 和 ELISA.
Rockland
产品编号 ABIN6656176
Supplier Product No.: 200-306-w56
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GFAP 抗体 (AA 411-422) (Biotin) (ABIN6656176)

抗原

See all GFAP 抗体
GFAP (Glial Fibrillary Acidic Protein (GFAP))

适用

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小鼠

克隆类型

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单克隆

标记

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This GFAP antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

克隆位点

S206B-9
  • 抗原表位

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    AA 411-422

    Supplier Product No.

    200-306-w56

    Supplier

    Rockland

    原理

    GFAP R416WT Antibody Biotin

    交叉反应 (详细)

    A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100 % homology with the immunizing sequence.

    纯化方法

    Anti-GFAP R416WT Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

    过滤

    Sterile filtered

    免疫原

    Anti-GFAP R416WT Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.

    亚型

    IgG1
  • 应用备注

    ELISA_Dilution: 1:10,000

    Immunohistochemistry_Dilution: User Optimized

    Western_Blot_Dilution: 1:1000

    说明

    Anti-GFAP R416WT Biotin Conjugated Antibody is suitable for use in Western blot, Immunohistochemistry, and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.1 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    GFAP (Glial Fibrillary Acidic Protein (GFAP))

    别名

    GFAP

    背景

    Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody

    Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.

    Gene Name: GFAP

    基因ID

    2670

    UniProt

    P14136
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