Choline Acetyltransferase 抗体
(1 reference)
Quick Overview for Choline Acetyltransferase 抗体 (ABIN6656077)
抗原
See all Choline Acetyltransferase (CHAT) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-101-d16
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Supplier
- Rockland
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原理
- ChAT (Choline Acetyltransferase) Antibody
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交叉反应 （详细）
- Anti-ChAT antibody is directed against human ChAT protein.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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免疫原
- Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.
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亚型
- IgG
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anti-Choline O-Acetyltransferase (CHAT) (AA 517-732) antibody
CHAT 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 331-630) antibodyCHAT 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 98-128) antibodyCHAT 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 101-200) antibodyCHAT 适用: 人, 小鼠, 大鼠, 犬 ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 354-592) antibodyCHAT 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (C-Term) antibodyVerified CHAT 适用: 小鼠, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) antibodyCHAT 适用: 人 WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 446-652) antibodyCHAT 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) antibodyMicroarray verified CHAT 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB872084 unconjugated
anti-Choline O-Acetyltransferase (CHAT) (C-Term) antibodyCHAT 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry_Dilution: 1:100
Western_Blot_Dilution: 1:100
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说明
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Anti-ChAT antibody is tested by western blot and immunohistochemistry and suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 5 mg BSA
Preservative: 0.02 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep." in: Nature, Vol. 548, Issue 7669, pp. 582-587, (2017) (PubMed).
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: "Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep." in: Nature, Vol. 548, Issue 7669, pp. 582-587, (2017) (PubMed).
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- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
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别名
- ChAT (Choline Acetyltransferase)
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背景
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Synonyms: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2
Background: ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Gene Name: CHAT
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基因ID
- 1103
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UniProt
- P28329
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途径
- Skeletal Muscle Fiber Development
抗原
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