UBE2M 抗体 (AA 169-180)
(2 references)
Quick Overview for UBE2M 抗体 (AA 169-180) (ABIN6656076)
抗原
See all UBE2M (ube2m) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 169-180
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Supplier Product No.
- 600-401-865
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Supplier
- Rockland
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原理
- UBC12 Antibody
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交叉反应 （详细）
- Reactivity occurs against human UBC12 protein.
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纯化方法
- UBC12 Antibody is produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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过滤
- Sterile filtered
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免疫原
- This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region near aa 150-183 of Human UBC12 protein.
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亚型
- IgG
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anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (N-Term) antibody
ube2m 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) antibodyube2m 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3C4 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (Internal Region) antibodyube2m 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 11-28) antibodyube2m 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) antibodyube2m 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2M (ube2m) (N-Term) antibodyube2m 适用: 人, 小鼠, 非洲爪蟾, 大鼠, 犬, Cow, 豚鼠, 马, 猴, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:2,000 - 1:10,000
Western_Blot_Dilution: 1:500 - 1:2,000
Other: User Optimized
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说明
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UBC12 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to UBC12 by western blotting in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression." in: Molecular cell, Vol. 69, Issue 5, pp. 773-786.e6, (2019) (PubMed).
: "Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling." in: Virology, Vol. 377, Issue 1, pp. 133-42, (2008) (PubMed).
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: "Cand1-Mediated Adaptive Exchange Mechanism Enables Variation in F-Box Protein Expression." in: Molecular cell, Vol. 69, Issue 5, pp. 773-786.e6, (2019) (PubMed).
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- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
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别名
- UBC12
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背景
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Synonyms: rabbit anti-UBC12 antibody, UBC-12, UBC 12, NEDD-8, NEDD 8, NEDD8 carrier protein antibody, NEDD8 conjugating enzyme Ubc12 antibody, NEDD8 protein ligase antibody, UBE2M antibody, Ubiquitin conjugating enzyme E2 M antibody
Background: UBC12 (also known as Ubiquitin-conjugating enzyme E2M, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, and Nedd8-conjugating enzyme Ubc12) is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBC12 is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/cullin.
Gene Name: UBE2M
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基因ID
- 9040, 4507791
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UniProt
- P61081
抗原
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