CRYAA 抗体
(1 reference)
Quick Overview for CRYAA 抗体 (ABIN6656059)
抗原
See all CRYAA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-e84
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Supplier
- Rockland
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原理
- Alpha A Crystallin Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Alpha A Crystallin from Human, Mouse, Cow, and Rat based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Alpha A Crystallin Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- Alpha Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha Crystallin protein.
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亚型
- IgG1
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anti-Crystallin, alpha A (CRYAA) antibody
CRYAA 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-Crystallin, alpha A (CRYAA) (AA 1-173) antibodyCRYAA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha A (CRYAA) antibodyCRYAA 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Crystallin, alpha A (CRYAA) (AA 77-106) antibodyCRYAA 适用: 人 WB 宿主: 兔 Polyclonal RB28544 unconjugated
anti-Crystallin, alpha A (CRYAA) antibodyCRYAA 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal 1H3-B8 unconjugated
anti-Crystallin, alpha A (CRYAA) antibody (HRP)CRYAA 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal 1H3-B8 HRP
anti-Crystallin, alpha A (CRYAA) antibody (FITC)CRYAA 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal 1H3-B8 FITC
anti-Crystallin, alpha A (CRYAA) antibody (Biotin)CRYAA 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal 1H3-B8 Biotin
anti-Crystallin, alpha A (CRYAA) antibody (PE)CRYAA 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal 1H3-B8 PE
anti-Crystallin, alpha A (CRYAA) antibody (APC)CRYAA 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal 1H3-B8 APC
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应用备注
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ELISA_Dilution: 1:200
Western_Blot_Dilution: 1:2000
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说明
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Anti-Alpha A Crystallin Antibody has been tested in WB and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa protein corresponding to the molecular mass of α-crystallin on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation." in: Experimental eye research, Vol. 226, pp. 109306, (2023) (PubMed).
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: "A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation." in: Experimental eye research, Vol. 226, pp. 109306, (2023) (PubMed).
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- CRYAA (Crystallin, alpha A (CRYAA))
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别名
- Alpha A Crystallin
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背景
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Synonyms: Heat shock protein beta4, Acry 1, CRYA1, CRYAA, HspB4, Alpha-crystallin A chain
Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance. In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA. Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases.
Gene Name: CRYAA
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基因ID
- 1409
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NCBI登录号
- NP_000385
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UniProt
- P02489
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途径
- M Phase
抗原
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