TJP1 抗体
(1 reference)
Quick Overview for TJP1 抗体 (ABIN6655949)
抗原
See all TJP1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-gu7
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Supplier
- Rockland
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原理
- ZO-1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human ZO-1.
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody
TJP1 适用: 人, 小鼠, 大鼠, Pig, Cow WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (C-Term) antibodyTJP1 适用: 人, 小鼠, 大鼠, Pig, Cow, 犬, 豚鼠, 兔, 马, 猴, 小鸡, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (N-Term) antibodyKO Validated TJP1 适用: 人 WB, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (Internal Region) antibodyVerified TJP1 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (AA 1600-1700) antibodyTJP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) antibodyTJP1 适用: 人 WB, IHC, ELISA, IF, FACS, FM, MA 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) antibodyTJP1 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (AA 1178-1527) antibodyTJP1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) antibodyTJP1 适用: 小鼠 WB, IHC, IP 宿主: 大鼠 Monoclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (AA 1-190) antibodyTJP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 10,000-1:50,000
Immunohistochemistry_Dilution: 1:100-1:200
Flow_Cytometry_Dilution: User Optimized
IF_Microscopy_Dilution: 10 μg/mL
Western_Blot_Dilution: 1:1000
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说明
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Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Virtual measurements of paracellular permeability and chronic inflammation via color coded pixel-wise T1 mapping." in: American journal of physiology. Renal physiology, Vol. 319, Issue 3, pp. F506-F514, (2020) (PubMed).
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: "Virtual measurements of paracellular permeability and chronic inflammation via color coded pixel-wise T1 mapping." in: American journal of physiology. Renal physiology, Vol. 319, Issue 3, pp. F506-F514, (2020) (PubMed).
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- TJP1 (Tight Junction Protein 1 (TJP1))
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别名
- ZO-1
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背景
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Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1
Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.
Gene Name: TJP1
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基因ID
- 7082
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NCBI登录号
- NP_003248
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UniProt
- Q07157
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途径
- Carbohydrate Homeostasis, Cell-Cell Junction Organization
抗原
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