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TJP1 抗体

This anti-TJP1 antibody is a 兔 多克隆 antibody detecting TJP1 in WB, IHC, ELISA, IF, FACS, FM 和 MA. Suitable for 人. This Primary Antibody has been cited in 1 publication.
Rockland
产品编号 ABIN6655949
Supplier Product No.: 600-401-gu7
发货至: 中国

Quick Overview for TJP1 抗体 (ABIN6655949)

抗原

See all TJP1 抗体
TJP1 (Tight Junction Protein 1 (TJP1))

适用

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宿主

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克隆类型

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多克隆

标记

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This TJP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Multiplex Assay (MA)
  • Supplier Product No.

    600-401-gu7

    Supplier

    Rockland

    原理

    ZO-1 Antibody

    交叉反应 (详细)

    This affinity purified antibody is directed against human ZO-1.

    纯化方法

    This product was affinity purified from monospecific antiserum by immunoaffinity purification.

    过滤

    Sterile filtered

    免疫原

    Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

    亚型

    IgG
  • 应用备注

    ELISA_Dilution: 10,000-1:50,000

    Immunohistochemistry_Dilution: 1:100-1:200

    Flow_Cytometry_Dilution: User Optimized

    IF_Microscopy_Dilution: 10 μg/mL

    Western_Blot_Dilution: 1:1000

    说明

    Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Singh, Zabbarova, Ikeda, Maranchie, Chermansky, Foley, Hitchens, Yoshimura, Kanai, Kaufman, Tyagi: "Virtual measurements of paracellular permeability and chronic inflammation via color coded pixel-wise T1 mapping." in: American journal of physiology. Renal physiology, Vol. 319, Issue 3, pp. F506-F514, (2020) (PubMed).

  • 抗原

    TJP1 (Tight Junction Protein 1 (TJP1))

    别名

    ZO-1

    背景

    Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1

    Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.

    Gene Name: TJP1

    基因ID

    7082

    NCBI登录号

    NP_003248

    UniProt

    Q07157

    途径

    Carbohydrate Homeostasis, Cell-Cell Junction Organization
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