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TJP1 抗体

Name: TJP1 抗体
SKU: ABIN6655949
Availability: OutOfStock

TJP1 适用: 人 WB, IHC, ELISA, IF, FACS, FM, MA 宿主: 兔 Polyclonal unconjugated

Western Blot of Rabbit anti-ZO-1 antibody Western Blot of Rabbit Anti-ZO-1 Antibody. (TJP1 抗体)

Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody. (TJP1 抗体)

Immunohistochemistry of Rabbit anti-ZO-1 antibody Immunohistochemistry of Rabbit anti-ZO-1 antibody. (TJP1 抗体)

IHC

Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody Immunofluorescence Microscopy of Rabbit anti-ZO-1 antibody. (TJP1 抗体)

Immunofluorescence microscopy of ZO-1 Immunofluorescence microscopy of Anti-ZO-1 in Caco-2 cells using FITC-conjugated Fluorescent anti-rabbit IgG for detection. (TJP1 抗体)

6 images

(1 reference)

产品编号 ABIN6655949

Supplier Product No.: 600-401-gu7

发货至: 中国

Datasheet as PDF

抗原 See all TJP1 抗体

TJP1 (Tight Junction Protein 1 (TJP1))
适用
57

36

34

18

17

8

5

5

3

3

1

1

1

1

1

1

1

1
人
宿主
54

3

1

1

1
兔
Compatible Secondaries
克隆类型
55

5
多克隆
标记
37

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TJP1 antibody is un-conjugated
应用范围
49

21

18

16

14

13

12

11

10

9

8

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Supplier Product No.

600-401-gu7

Supplier

Rockland

原理

ZO-1 Antibody

交叉反应（详细）

This affinity purified antibody is directed against human ZO-1.

纯化方法

This product was affinity purified from monospecific antiserum by immunoaffinity purification.

过滤

Sterile filtered

免疫原

Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

亚型

IgG

Top Product

Discover our top product TJP1 Primary Antibody
anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody
TJP1 适用: 人, 小鼠, 大鼠, Pig, Cow WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (C-Term) antibody
TJP1 适用: 人, 小鼠, 大鼠, Pig, Cow, 犬, 豚鼠, 兔, 马, 猴, 小鸡, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (N-Term) antibody
KO Validated TJP1 适用: 人 WB, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (Internal Region) antibody
Verified TJP1 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 适用: 人 WB, IHC, ELISA, IF, FACS, FM, MA 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1178-1527) antibody
TJP1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 适用: 小鼠 WB, IHC, IP 宿主: 大鼠 Monoclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1-190) antibody
TJP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 23-502) antibody
TJP1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA_Dilution: 10,000-1:50,000
Immunohistochemistry_Dilution: 1:100-1:200
Flow_Cytometry_Dilution: User Optimized
IF_Microscopy_Dilution: 10 μg/mL
Western_Blot_Dilution: 1:1000

说明

Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Singh, Zabbarova, Ikeda, Maranchie, Chermansky, Foley, Hitchens, Yoshimura, Kanai, Kaufman, Tyagi: "Virtual measurements of paracellular permeability and chronic inflammation via color coded pixel-wise T1 mapping." in: American journal of physiology. Renal physiology, Vol. 319, Issue 3, pp. F506-F514, (2020) (PubMed).
抗原

TJP1 (Tight Junction Protein 1 (TJP1))

别名

ZO-1 (TJP1 产品)

背景

Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1
Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.
Gene Name: TJP1

基因ID

7082

NCBI登录号

NP_003248

UniProt

Q07157

途径

Carbohydrate Homeostasis, Cell-Cell Junction Organization