KLF4 抗体 (N-Term)
Quick Overview for KLF4 抗体 (N-Term) (ABIN6655945)
抗原
See all KLF4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 600-401-gu2s
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Supplier
- Rockland
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原理
- KLF4 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human KLF4.
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-KLF4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human KLF4 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Kruppel-Like Factor 4 (Gut) (KLF4) (C-Term) antibody
KLF4 适用: 人, 小鼠 WB, FACS, IP 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibodyKLF4 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibodyKLF4 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Monoclonal 3A1 unconjugated Recombinant Antibody
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 221-320) antibodyKLF4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal X1 unconjugated
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibodyKLF4 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14263 unconjugated
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (C-Term) antibodyKLF4 适用: 人, 小鼠, 大鼠, Cow, 马, 犬, 兔, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 69-101) antibodyKLF4 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 481-504) antibodyKLF4 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 4 (Gut) (KLF4) antibodyKO Validated KLF4 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 4 (Gut) (KLF4) (AA 394-421) antibodyKLF4 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: 5 μg/mL
Immunohistochemistry_Dilution: 1:200
Flow_Cytometry_Dilution: User Optimized
IF_Microscopy_Dilution: 10 μg/mL
Western_Blot_Dilution: 1 μg/mL
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说明
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Anti-KLF4 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at ~54.7 kDa in western blot using appropriate lysates. Positive control whole cell lysates NCCIT and PC3 @ 1μg/mL for WB, and PC3 with PFA at 10μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))
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别名
- KLF4
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背景
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Synonyms: rabbit anti-KLF4 Antibody, KLF-4 Antibody, Krueppel-like factor 4, Epithelial zinc finger protein EZF, EZF, Gut-enriched krueppel-like factor, GKLF, KLF 4
Background: KLF4 belong to the Kruppel like factors/Zinc fingers C2H2-type family. This gene encodes a protein that belongs to the Kruppel family of transcription factors. KLF4 can act both as activator and as repressor. It can bind the 5-CACCC-3 core sequence, the promoter region of its own gene, and can activate its own transcription. KLF4 regulates the expression of key transcription factors during embryonic development, playing an important role in maintaining embryonic stem cells, and in preventing their differentiation. This protein is required for normal development of the barrier function of skin postnatal, maintenance of the ocular surface, function in skeletal and kidney development, and thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. KLF4 may be associated with diseases such as Venous Hemangioma, Skin Squamous Cell Carcinoma, and pediatric lymphoma. Anti-KLF4 Antibody is useful for researchers interested in Peptide hormone metabolism, Stem Cell Differentiation Pathway Research, Wnt/Notch Pathway Research, and Cancer Research.
Gene Name: KLF4
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基因ID
- 9314
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NCBI登录号
- NP_001300981
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UniProt
- O43474
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途径
- Peptide Hormone Metabolism, Stem Cell Maintenance
抗原
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