HIST1H3A 抗体 (H3K27me)
Quick Overview for HIST1H3A 抗体 (H3K27me) (ABIN6655652)
抗原
See all HIST1H3A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- H3K27me
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Supplier Product No.
- 600-401-w54
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Supplier
- Rockland
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原理
- Histone H3 K27me1 Antibody
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交叉反应 （详细）
- This product reacts with human Histone 3 methyl Lys27.
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纯化方法
- Anti-Histone H3 k27 me1 Antibody was purified by affinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-Histone H3 K27 me1 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of histone H3 containing the monomethylated lysine 27.
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亚型
- IgG
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anti-Histone Cluster 1, H3a (HIST1H3A) (acLys27) antibody
HIST1H3A 适用: 人 ELISA, WB, IF, ICC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) antibodyHIST1H3A 适用: 人 ELISA, WB, IHC, IF, FACS 宿主: 兔 Monoclonal 27F2 unconjugated Recombinant Antibody
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys4) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (H3K4me2) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ICC, FACS 宿主: 兔 Monoclonal 6F6 unconjugated Recombinant Antibody
anti-Histone Cluster 1, H3a (HIST1H3A) (pSer10) antibodyHIST1H3A 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (H3K27me2) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ChIP, DB, FM 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys9) antibodyHIST1H3A 适用: 人 ELISA, WB, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys37) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys36) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Histone Cluster 1, H3a (HIST1H3A) (acLys23) antibodyHIST1H3A 适用: 人 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ChIP_Dilution: 1 μg per IP
ELISA_Dilution: 1:500
IF_Microscopy_Dilution: 1:1,000
Western_Blot_Dilution: 1:1,000
Other: Dot Blot: 1:20,000
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说明
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Anti-Histone H3 K27 me1 Antibody is tested in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15kDa in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300 -
储存液
- ProClin, Sodium azide
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注意事项
- This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
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别名
- HIST1H3A
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背景
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Synonyms: Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/e, Histone H3/f, Histone H3/g, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called ""histone code"". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Anti-Histone H3 K27 me1 Antibody is ideal for research in Chromatin Remodeling and Genetics.
Gene Name: HIST1H3A
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基因ID
- 3020
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NCBI登录号
- NP_002098
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UniProt
- P84243
抗原
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