CES1 抗体
Quick Overview for CES1 抗体 (ABIN6655642)
抗原
See all CES1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-b14s
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Supplier
- Rockland
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原理
- Liver Carboxylesterase-1 Antibody
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交叉反应 （详细）
- The antibody detects recombinant hCE1 in cell lysates and native Liver Carboxylesterase-1 present in human tissue or fluids in various assays (ie.
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纯化方法
- This product was prepared from monospecific antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
- This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.
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亚型
- IgG
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anti-Carboxylesterase 1 (CES1) (AA 19-300) antibody
CES1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 1 (CES1) (AA 18-299) antibodyCES1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 1 (CES1) (AA 291-566) antibodyCES1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 1 (CES1) (AA 287-566) antibodyCES1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 1 (CES1) antibodyCES1 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Carboxylesterase 1 (CES1) (AA 99-206) antibodyCES1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 1 (CES1) (AA 31-286) antibodyCES1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 1 (CES1) (Internal Region) antibodyVerified CES1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Carboxylesterase 1 (CES1) (AA 1-566) antibodyCES1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 1 (CES1) (AA 1-566) antibodyCES1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunoprecipitation_Dilution: 1:100
ELISA_Dilution: 1:5,000 - 1:20,000
Western_Blot_Dilution: 1:2,500 - 1:5,000
Other: User Optimized
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说明
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This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66 kDa in size corresponding CE1 by western blotting in the appropriate cell lysate or extract. In western blot analysis endogenous protein present in human tissue or fluids, multiple bands of CE may appear corresponding to variable amount of glycosylation on the molecule.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 3 months
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- CES1 (Carboxylesterase 1 (CES1))
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别名
- CES1
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背景
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Synonyms: rabbit anti-Liver Carboxylesterase-1 Antibody, Liver carboxylesterase 1, Acyl coenzyme A:cholesterol acyltransferase, ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, Egasyn, Retinyl ester hydrolase, REH
Background: Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.
Gene Name: CES1
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基因ID
- 1066, 68508965
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UniProt
- P23141
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途径
- Monocarboxylic Acid Catabolic Process
抗原
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