CD86 抗体 (PE)
Quick Overview for CD86 抗体 (PE) (ABIN6655468)
抗原
See all CD86 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-308-n75
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Supplier
- Rockland
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原理
- CD86 Phycoerythrin Antibody
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交叉反应 (详细)
- Reactivity is observed against human CD86.
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纯化方法
- Phycoerythrin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD86.
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过滤
- Sterile filtered
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免疫原
- Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.
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亚型
- IgG2b kappa
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Labeling Ratio
- 1-2
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应用备注
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Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells
Western_Blot_Dilution: User Optimized
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说明
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Anti-CD86 is tested for Flow Cytometry and useful in Immunohistochemistry, and Western Blot. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 6 months
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- CD86
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别名
- CD86
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背景
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Synonyms: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD28LG2
Background: CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Gene Name: CD86
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基因ID
- 942
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NCBI登录号
- NP_001193853
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UniProt
- P42081
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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