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CD86 抗体 (PE)

This anti-CD86 antibody is a 小鼠 单克隆 antibody detecting CD86 in FACS, WB 和 IHC. Suitable for 人.
Rockland
产品编号 ABIN6655468
Supplier Product No.: 200-308-n75
发货至: 中国

Quick Overview for CD86 抗体 (PE) (ABIN6655468)

抗原

See all CD86 抗体
CD86

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD86 antibody is conjugated to PE

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

IT2-2
  • Supplier Product No.

    200-308-n75

    Supplier

    Rockland

    原理

    CD86 Phycoerythrin Antibody

    交叉反应 (详细)

    Reactivity is observed against human CD86.

    纯化方法

    Phycoerythrin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD86.

    过滤

    Sterile filtered

    免疫原

    Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.

    亚型

    IgG2b kappa

    Labeling Ratio

    1-2
  • 应用备注

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

    Western_Blot_Dilution: User Optimized

    说明

    Anti-CD86 is tested for Flow Cytometry and useful in Immunohistochemistry, and Western Blot. Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.2 % BSA (w/v)

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

    有效期

    6 months
  • 抗原

    CD86

    别名

    CD86

    背景

    Synonyms: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD28LG2

    Background: CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

    Gene Name: CD86

    基因ID

    942

    NCBI登录号

    NP_001193853

    UniProt

    P42081

    途径

    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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