CD86 抗体 (APC)
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- 抗原 See all CD86 抗体
- CD86
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD86 antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 200-326-n75
- Supplier
- Rockland
- 原理
- CD86 Allophycocyanin Antibody
- 交叉反应 (详细)
- Reactivity is observed against human CD86.
- 纯化方法
- Allophycocyanin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD86.
- 过滤
- Sterile filtered
- 免疫原
- Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.
- 克隆位点
- IT2-2
- 亚型
- IgG2b kappa
- Labeling Ratio
- 1-2
- Top Product
- Discover our top product CD86 Primary Antibody
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- 应用备注
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Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells
Western_Blot_Dilution: User Optimized
- 说明
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Anti-CD86 is tested for Flow Cytometry and useful in Immunohistochemistry and Western Blot. Researchers should determine optimal titers for applications that are not stated.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
Preservative: 0.09 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
- 有效期
- 6 months
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- 抗原
- CD86
- 别名
- CD86 (CD86 产品)
- 背景
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Synonyms: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD28LG2
Background: CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Gene Name: CD86
- 基因ID
- 942
- NCBI登录号
- NP_001193853
- UniProt
- P42081
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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