CD8 alpha 抗体 (PE)
Quick Overview for CD8 alpha 抗体 (PE) (ABIN6655405)
抗原
See all CD8 alpha (CD8A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-308-n62
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Supplier
- Rockland
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原理
- CD8a Phycoerythrin Antibody
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交叉反应 （详细）
- Reactivity is observed against human CD8a.
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纯化方法
- Phycoerythrin conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD8a.
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过滤
- Sterile filtered
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免疫原
- Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.
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亚型
- IgG2a
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Labeling Ratio
- 1-2
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anti-CD8a Molecule (CD8A) (AA 22-235) antibody
CD8A 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 8H8A8 unconjugated
anti-CD8a Molecule (CD8A) (AA 51-150) antibodyCD8A 适用: 人, 大鼠, 小鸡 FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, WB, IP, ELISA 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, ICC, IHC (fro), IHC (p), Func 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, ICC, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal C8-468 unconjugated
anti-CD8a Molecule (CD8A) (AA 22-182) antibodyCD8A 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6A9A5 unconjugated
anti-CD8a Molecule (CD8A) (AA 28-196) antibodyCD8A 适用: 小鼠, 大鼠 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) (AA 150-180) antibodyCD8A 适用: 人 FACS, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) (AA 59-88) antibodyCD8A 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells
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说明
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Anti-CD8a is tested in Flow Cytometry and is useful for Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.1 % Gelatin
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
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有效期
- 6 months
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- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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别名
- CD8a
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背景
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Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a
Background: The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The OKT8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
Gene Name: CD8A
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基因ID
- 925
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NCBI登录号
- NP_001139345
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UniProt
- P01732
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途径
- TCR Signaling
抗原
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