CD8 alpha 抗体 (FITC)
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北京 101111
Quick Overview for CD8 alpha 抗体 (FITC) (ABIN6655402)
抗原
See all CD8 alpha (CD8A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-302-n61
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Supplier
- Rockland
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原理
- CD8a Fluorescein Antibody
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交叉反应 (详细)
- Reactivity is observed against human CD8a, Marmoset, and chimpanzee.
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纯化方法
- Fluorescein conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD8a.
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过滤
- Sterile filtered
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免疫原
- Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.
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亚型
- IgG1 kappa
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Labeling Ratio
- 4-6
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应用备注
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Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood
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说明
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Anti-CD8a has been tested in Flow Cytometry and is useful for Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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有效期
- 6 months
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- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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别名
- CD8a
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背景
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Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a
Background: CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Gene Name: CD8A
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基因ID
- 925
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NCBI登录号
- NP_001139345
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UniProt
- P01732
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途径
- TCR Signaling
抗原
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