CTSA 抗体
Quick Overview for CTSA 抗体 (ABIN6655368)
抗原
See all CTSA 抗体适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 100-401-395
-
Supplier
- Rockland
-
原理
- Protective Protein/Cathepsin A Antibody
-
交叉反应 （详细）
- Based on sequence we expect this antibody to react with PPCA from other sources, although specific reactivity has not been confirmed.
-
纯化方法
- This antiserum is directed against PPCA and reacts with the PPCA from human tissues.
-
过滤
- Sterile filtered
-
免疫原
- This whole rabbit serum was prepared by repeated immunizations with native 54-kDa human Protective Protein/Cathepsin A (PPCA) precursor, purified from the medium of baculovirus infected insect cells.
-
亚型
- IgG
-
-
anti-Cathepsin A (CTSA) (AA 18-45), (N-Term) antibody
CTSA 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB23380 unconjugated
anti-Cathepsin A (CTSA) (AA 215-470) antibodyCTSA 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin A (CTSA) (AA 401-480) antibodyCTSA 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin A (CTSA) (AA 30-327) antibodyCTSA 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin A (CTSA) (C-Term) antibodyCTSA 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin A (CTSA) (AA 215-470) antibodyCTSA 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin A (CTSA) (AA 25-53), (N-Term) antibodyCTSA 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin A (CTSA) (Cleaved-Arg326) antibodyCTSA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin A (CTSA) (AA 234-494) antibodyCTSA 适用: 犬 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin A (CTSA) (AA 1-480) antibodyCTSA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
-
-
应用备注
-
ELISA_Dilution: 1:1,000 - 1:5,000
Immunohistochemistry_Dilution: 1:500
Western_Blot_Dilution: 1:500 - 1:2,000
Other: User Optimized
-
说明
-
Suggested Applications: IP
Anti-PPCA has been tested by immunohistochemistry and is suitable for the detection of human PPCA (54-, 32-, and 20-kDa) on Western blot, by immunoprecipitation, immunocytochemistry, and ELISA of either transfected cells (e.g. COS-1 cells) or primary cells (e.g. fibroblasts). -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- CTSA (Cathepsin A (CTSA))
-
别名
- Protective Protein/Cathepsin A
-
背景
-
Synonyms: rabbit anti-Protective Protein Antibody, rabbit anti-Cathepsin A Antibody, Beta Galactosidase Protective Protein antibody, Carboxypeptidase C antibody, Cathepsin A antibody, CTSA antibody, Glactosialidosis antibody, GLB2 antibody, Goldberg Syndrome antibody
Background: Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54- kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20- kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH . Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.
Gene Name: CTSA, PPGB
-
基因ID
- 5476
-
UniProt
- P10619
抗原
-
