MITF 抗体
Quick Overview for MITF 抗体 (ABIN6655359)
抗原
See all MITF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-h93
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Supplier
- Rockland
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原理
- MITF Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with MITF from Human, Rat, and Mouse based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-MITF Antibody was purified by Protein G chromatography.
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免疫原
- MITF Antibody was produced in mice prepared by repeated immunizations with a synthetic peptide (sequence PSTGLCSPDLVN) corresponding to internal amino acids with human MITF protein.
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亚型
- IgG1
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anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 351-450) antibody
MITF 适用: 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer180), (pSer73) antibodyMITF 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-114) antibodyMITF 适用: 人 WB, FACS, ELISA 宿主: 小鼠 Monoclonal 8F1G5 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-28), (N-Term) antibodyMITF 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18810 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人, 犬 IHC, IF, FACS, StM 宿主: 兔 Monoclonal MITF-2987R unconjugated Recombinant Antibody
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-280) antibodyMITF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人, 小鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal D5 unconjugated
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应用备注
- Optional[Neutralization_Dilution]: 3-5 μg/mL
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说明
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Anti-MITF antibody is tested for WB, IHC, and IF. Expect a band approximately 52kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.05 % BSA
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MITF (Microphthalmia-Associated Transcription Factor (MITF))
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别名
- MITF
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背景
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Synonyms: BHLHE32, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
Background: Anti-MITF antibody detects human MITF. Microphthalmia-associated bHLH-LZ transcription factor (MITF) is a positively acting transcription factor containing a transcription activation domain and a bHLH-LZ domain that mediates DNA-binding and dimerization. Both the transcription activation and bHLH-LZ domains are highly conserved in the closely related TFE3, TFEB and TFEC. MITF can form heterodimers in vitro with the above transcription factors, but not with other bHLH-LZ factors such as Myc, Max or USF. MITF is expressed as multiple isoforms, which share common activation and DNA-binding domains, but differ in the amino-terminal region. MITF is targeted by three kinases: GSK-3beta, Map kinase ERK2 and members of the p90 Rsk family of kinases. Anti-MITF antibody is ideal for investigators involved in kinase, phosphatase, NFkappaB, and signaling research.
Gene Name: MITF
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基因ID
- 4286
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NCBI登录号
- NP_000239
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UniProt
- O75030
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途径
- Chromatin Binding
抗原
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