DNAJB1 抗体
Quick Overview for DNAJB1 抗体 (ABIN6655346)
抗原
See all DNAJB1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 109-401-e57
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Supplier
- Rockland
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原理
- HSP40 (HDJ1) Antibody
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交叉反应 (详细)
- This polyclonal antibody is specific for human Hsp40 protein.
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纯化方法
- Anti-Hsp40 Antibody was prepared from monospecific antiserum by delipidation and defibrination.
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过滤
- Sterile filtered
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免疫原
- Hsp40 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against recombinant purified human hsp40.
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亚型
- IgG
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Western_Blot_Dilution: 1: 1500-2,000
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说明
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Anti-Hsp40 Antibody has been tested in WB, IP, and IF. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Also detects recombinant Hsp40. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))
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别名
- Hsp40
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背景
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Synonyms: Rabbit anti-Hsp40 Antibody, rabbit anti-heat shock protein 40 antibody, Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1, YDJ1, MAS5, YNL064C, N2418, YNL2418C
Background: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1, is a basic mammalian 40 kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1. Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK.
Gene Name: YDJ1
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基因ID
- 3337
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NCBI登录号
- NP_006136
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UniProt
- P25685
抗原
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