CD47 抗体 (FITC)
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北京 101111
Quick Overview for CD47 抗体 (FITC) (ABIN6655123)
抗原
See all CD47 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-302-m68
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Supplier
- Rockland
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原理
- CD47 Fluorescein Antibody
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交叉反应 （详细）
- Reactivity is observed against human CD47.
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纯化方法
- Fluorescein conjugated CD47 Monoclonal Antibody was Protein G Purified and is directed against human CD47.
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过滤
- Sterile filtered
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免疫原
- Anti-CD47 Antibody (Monoclonal) was produced by repeated immunizations with CD47 antigen.
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亚型
- IgG1
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Labeling Ratio
- 2-8
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anti-CD47 (CD47) (AA 20-140) antibody
CD47 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) antibodyCD47 适用: 人, 小鼠 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal B6H12-2 unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 适用: 人 FACS, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal CD47-3019 unconjugated
anti-CD47 (CD47) (AA 241-323) antibodyCD47 适用: 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-219) antibodyCD47 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 适用: 人 FACS, WB, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal CD47-2937 unconjugated
anti-CD47 (CD47) antibody (FITC)CD47 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-122 FITC
anti-CD47 (CD47) antibodyCD47 适用: 人, Pig, Non-Human Primate FACS, WB, IHC (fro) 宿主: 小鼠 Monoclonal MEM-122 unconjugated
anti-CD47 (CD47) antibodyCD47 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Monoclonal 4G11 unconjugated Recombinant Antibody
anti-CD47 (CD47) (AA 44-141) antibodyCD47 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry_Dilution: User Optimized
Flow_Cytometry_Dilution: 10 μL/5 x 10^5 cells (0.5 μg)
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说明
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Anti-CD47 has been tested in Flow Cytometry (Cell Surface) and is useful for Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Optional[Buffer]: 0.01 M Tris Cl, 0.15 M Sodium Chloride, pH 8.0 ,0.05 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
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有效期
- 6 months
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- CD47
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别名
- CD47
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背景
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Synonyms: Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47
Background: CD47, originally named integrin-associated protein (IAP), is a 50- kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-a, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.
Gene Name: CD47
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基因ID
- 961
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NCBI登录号
- NP_001768
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UniProt
- Q08722
抗原
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