KDELR1 抗体
Quick Overview for KDELR1 抗体 (ABIN6655048)
抗原
See all KDELR1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 500-301-f86
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Supplier
- Rockland
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原理
- KDEL Receptor Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with KDEL from Human, Monkey, Rat, Mouse, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, and Xenopus based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-KDEL Antibody is clarified mouse ascites fluid.
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过滤
- Sterile filtered
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免疫原
- KDEL Receptor Antibody was produced in mice by repeated immunizations raised against a 21 residue synthetic peptide based on the bovine KDEL receptor and the synthetic peptide coupled to KLH.
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亚型
- IgG1
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anti-KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1) (Internal Region) antibody
KDELR1 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1) (AA 192-212) antibodyKDELR1 适用: 人, 小鼠, 大鼠, Cow, 犬, 猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western_Blot_Dilution: 1:1000
Other: ICC/IF: 1:1000
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说明
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Anti-KDEL Antibody is tested for WB and IF microscopy and suitable for IP and IHC. Expect a band approximately ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))
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别名
- KDELR1
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背景
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Synonyms: mouse anti-KDEL Receptor Antibody, ERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23, Lys Asp Glu Leu, K-D-E-L, ER lumen protein retaining receptor 1, KDEL endoplasmic reticulum protein retention, Kdelr1
Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention. There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments. In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding. It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal.
Gene Name: Kdelr1
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基因ID
- 618184
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NCBI登录号
- NP_001069963
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UniProt
- P33946
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途径
- Maintenance of Protein Location
抗原
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