Recombinant ANO1 抗体 (AA 2-101)
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Quick Overview for Recombinant ANO1 抗体 (AA 2-101) (ABIN6654568)
抗原
See all ANO1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 2-101
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原理
- Recombinant DOG1 Antibody (azide and preservative free)
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纯化方法
- Protein A affinity chromatography
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免疫原
- A portion of amino acids 2-101 from the human protein was used as the immunogen for this recombinant DOG1 antibody.
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亚型
- IgG, kappa
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant DOG1 antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant DOG1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))
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别名
- DOG1
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背景
- Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST's is nearly identical: 94.4 % vs. 94.7 %.
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UniProt
- Q5XXA6
抗原
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