The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Spectrin beta III antibody to be titered up or down for optimal performance.\. Western blot: 0.5-1 μg/mL,Flow cytometry: 1-2 μg/10^6 cells in 0.1ml,Immunofluorescence: 1-2 μg/mL
限制
仅限研究用
缓冲液
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store the recombinant Spectrin beta III antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
抗原
Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)
别名
SPTBN2 / Spectrin beta III
背景
Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.