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Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) 抗体

The 兔 单克隆 anti-Spectrin, Beta, Non-erythrocytic 2 antibody (Clone SPTBN2-2894R) (ABIN6654518) specifically detects Spectrin, Beta, Non-erythrocytic 2 in WB, FACS 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN6654518
发货至: 中国
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Quick Overview for Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) 抗体 (ABIN6654518)

抗原

See all Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) 抗体
Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

适用

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  • 1

宿主

  • 67
  • 42

克隆类型

  • 99
  • 10
单克隆

标记

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  • 9
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非结合性

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SPTBN2-2894R
  • 抗原表位

    • 97
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 356-475

    纯化方法

    Purified

    纯度

    Protein A affinity chromatography

    免疫原

    Human recombinant partial protein corresponding to amino acids 356-475 was used as the immunogen for this recombinant Spectrin beta III antibody.

    亚型

    IgG kappa
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Spectrin beta III antibody to be titered up or down for optimal performance.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Flow cytometry: 1-2 μg/10^6 cells in 0.1ml,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    限制

    仅限研究用
  • 缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant Spectrin beta III antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

    别名

    SPTBN2 / Spectrin beta III

    背景

    Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta.

    途径

    Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis
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