Optimal dilution of the recombinant ECM1 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
限制
仅限研究用
缓冲液
1 mg/mL in 1X PBS, BSA free, sodium azide free
储存液
Azide free
储存条件
4 °C,-20 °C
储存方法
Store the recombinant ECM1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
抗原
ECM1
(Extracellular Matrix Protein 1 (ECM1))
别名
Secretory Component p85 / ECM1
背景
This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.