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- 抗原
- SMN1 / SMN2
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适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 纯化方法
- Protein G affinity
- 免疫原
- Amino acids RRGTGQSDDSDIWDDTALIKAYDKAVASFKH were used as the immunogen for the SMN1/2 antibody.
- 克隆位点
- 2B10
- 亚型
- IgG1
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anti-SMN1 / SMN2 (AA 22-52), (N-Term) antibody
适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SMN1 / SMN2 (Internal Region) antibodyVerified 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-SMN1 / SMN2 (AA 51-100) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SMN1 / SMN2 (AA 22-52), (N-Term) antibody适用: 人 WB 宿主: 小鼠 Monoclonal 2B10 unconjugated
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- 应用备注
- Optimal dilution of the SMN1/2 antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL,ICC: 0.5-1 μg/mL
- 限制
- 仅限研究用
-
- 缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- 储存条件
- -20 °C
- 储存方法
- After reconstitution, the SMN1/2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
-
- 抗原
- SMN1 / SMN2
- 别名
- SMN1/2
- 背景
- This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein. However, mutations in this gene, the telomeric copy, are associated with spinal muscular atrophy, mutations in the centromeric copy do not lead to disease. The centromeric copy may be a modifier of disease caused by mutation in the telomeric copy. The critical sequence difference between the two genes is a single nucleotide in exon 7, which is thought to be an exon splice enhancer. Note that the nine exons of both the telomeric and centromeric copies are designated historically as exon 1, 2a, 2b, and 3-8. It is thought that gene conversion events may involve the two genes, leading to varying copy numbers of each gene. The protein encoded by this gene localizes to both the cytoplasm and the nucleus. Within the nucleus, the protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). This protein forms heteromeric complexes with proteins such as SIP1 and GEMIN4, and also interacts with several proteins known to be involved in the biogenesis of snRNPs, such as hnRNP U protein and the small nucleolar RNA binding protein. Multiple transcript variants encoding distinct isoforms have been described.
- UniProt
- Q16637
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