Immunoblotting: use at 0.5-2 μg/mL. Predicted molecular weight ~100 kDa. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
限制
仅限研究用
状态
Liquid
溶解方式
Dilute in PBS or medium which is identical to that used in the assay system.
浓度
1.0 mg/mL
缓冲液
Hybridoma culture supernatant
储存条件
-20 °C
储存方法
This antibody is stable for at least one year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
抗原
TSG101
(Tumor Susceptibility Gene 101 (TSG101))
别名
TSG101
背景
DNA ligase 3,Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627). {PubMed:10207110, PubMed:24674627}.,There are four distinct ligase proteins found in mammalian cells. These DNA ligases are required for joining DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III is a 100 kDa (a-form) protein in somatic tissues, is ubiquitously expressed, and complexes with XRCC-1 and a 97 kDa ( -form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in base excision repair pathway.,[Isoform 1]: Mitochondrion,EC 6.5.1.1, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3