RabbitBP4 / RabbitAP48 (AA 1-425) 抗体
The 小鼠 单克隆 anti-RabbitBP4 / RabbitAP48 antibody (Clone 15G12) (ABIN189975) specifically detects RabbitBP4 / RabbitAP48 in IF/ICC, IHC (p), IP, WB 和 EMSA.
The antibody is reactive with 人 samples.
产品编号 ABIN189975
发货至:
中国
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Quick Overview for RabbitBP4 / RabbitAP48 (AA 1-425) 抗体 (ABIN189975)
抗原
RabbitBP4 / RabbitAP48
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB), Electrophoretic Mobility-Shift Assay (EMSA)
克隆位点
15G12
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抗原表位
- AA 1-425
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原理
- Anti-RbAp48 Mouse Monoclonal Antibody
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特异性
- This antibody recognizes human and mouse RbAp48 and RbAp46.
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交叉反应
- 人
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免疫原
- GST fusion protein corresponding to aa 1-425 of human RbAp48.
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亚型
- IgG2a
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应用备注
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Immunoblotting: use at 1-5 μg/mL. A band of ~48 kDa is detected. Detection of RbAp48 in K562, THP-1, and HL-60 whole cell extracts (30 μg), separated by 10 % SDS-PAGE, with #3111 at 2 μg/mL.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.
Immunohistochemistry: use at 5-10 μg/mL. Detection of RbAp48 in paraffin-embedded human cervical carcinoma with #3111 at 10 μg/mL. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Dilute in PBS or medium which is identical to that used in the assay system.
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4
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储存条件
- -20 °C
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储存方法
- This antibody is stable at -20°C for at least one (1) year, store in appropriate aliquots to avoid multiple freeze-thaw cycles.
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- RabbitBP4 / RabbitAP48
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背景
- Retinoblastoma-associated protein,Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}., (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.,Nucleus,p105-Rb, p110-RB1, pRb, Rb, pp110
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基因ID
- 5925
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NCBI登录号
- NP_000312
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UniProt
- P06400
抗原
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