CD172a/b 抗体 (PE)
This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS. Suitable for 人.
Quick Overview for CD172a/b 抗体 (PE) (ABIN6559829)
抗原
CD172a/b
适用
人
宿主
小鼠
克隆类型
单克隆
标记
PE
应用范围
Flow Cytometry (FACS)
克隆位点
SE5A5
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原理
- Anti-Hu CD172ab PE
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特异性
- The mouse monoclonal antibody SE5A5 recognizes a common extracellular epitope on human CD172a and CD172b antigens (approx. 90 kDa and approx. 50 kDa, respectively), although its reactivity with CD172a is higher.
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交叉反应 （详细）
- Human
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纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- NIH-3T3 / human CD172a cell line
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亚型
- IgG1
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anti-CD172a/b antibody (APC)
适用: 人 FACS 宿主: 小鼠 Monoclonal SE5A5 APC
anti-CD172a/b antibody适用: 人 FACS 宿主: 小鼠 Monoclonal SE5A5 unconjugated
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
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限制
- 仅限研究用
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- CD172a/b
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别名
- CD172ab
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背景
- CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells. Extracellular part of CD172b is 90 % identical to that of CD172a, but unlike CD172, it has dramatically reduced intracellular domain.,PTPNS1, BIT, MFR, SIRPA, SHPS1, SIRPB1,
抗原
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