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WT1 抗体 (AA 346-375)

This anti-WT1 antibody is a 兔 多克隆 antibody detecting WT1 in IF, FACS, WB 和 IHC (p). Suitable for 人 和 小鼠.
产品编号 ABIN655904
发货至: 中国

Quick Overview for WT1 抗体 (AA 346-375) (ABIN655904)

抗原

See all WT1 抗体
WT1 (Wilms Tumor 1 (WT1))

适用

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人, 小鼠

宿主

  • 105
  • 70
  • 1

克隆类型

  • 99
  • 77
多克隆

标记

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This WT1 antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB18841
  • 抗原表位

    • 34
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    AA 346-375

    预测反应

    X, Pig, Rat

    纯化方法

    This antibody is purified through a protein A column, followed by peptide affinity purification.

    免疫原

    This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1.

    亚型

    IgG
  • 应用备注

    IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:25

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

    有效期

    6 months
  • 抗原

    WT1 (Wilms Tumor 1 (WT1))

    别名

    WT1

    背景

    This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].

    分子量

    49188

    基因ID

    7490

    NCBI登录号

    NP_000369, NP_001185480, NP_001185481, NP_077742, NP_077744

    UniProt

    P19544

    途径

    Tube Formation
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