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SMAD2 抗体 (pThr220)

Name: SMAD2 抗体 (pThr220)
SKU: ABIN650816
Availability: OutOfStock

SMAD2 适用: 人 WB, IF, DB 宿主: 兔 Polyclonal RB18943 unconjugated

Western blot analysis of lysates from Hela cell line, untreated or treated with TGF-β, 100 ng/mL, 30 min, using Phospho-SD2 Antibody (ABIN650816 and ABIN2839794) (upper) or Beta-actin (lower). (SMAD2 抗体 (pThr220))

Confocal immunofluorescent analysis of Phospho-SD2- Antibody (ABIN650816 and ABIN2839794) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (SMAD2 抗体 (pThr220))

Dot blot analysis of anti-Phospho-SD2- Phospho-specific Pab (ABIN650816 and ABIN2839794)? (SMAD2 抗体 (pThr220))

3 images

产品编号 ABIN650816

发货至: 中国

Datasheet as PDF

抗原 See all SMAD2 抗体

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
抗原表位
45

37

28

25

21

16

9

9

7

7

7

6

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr220
适用
228

147

114

12

10

9

9

8

7

7

7

6

5

4

3

3

1

1
人
宿主
231

10
兔
Compatible Secondaries
克隆类型
208

33
多克隆
标记
119

18

12

10

8

8

8

8

8

7

7

5

5

5

5

5

3
This SMAD2 antibody is un-conjugated
应用范围
203

99

62

53

52

42

40

39

17

14

9

9

5

2

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

预测反应

B, Zf, M, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This SMAD2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T220 of human SMAD2.

克隆位点

RB18943

亚型

Ig Fraction

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anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
SMAD2 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5G7 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) antibody
SMAD2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer423), (pSer425), (pSer465), (pSer467) antibody
SMAD2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
KO Validated SMAD2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (C-Term) antibody
SMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D10 unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer467) antibody
SMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibody
KO Validated SMAD2 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pThr183), (pThr221) antibody
SMAD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:500. DB: 1:500

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
抗原

SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

别名

SMAD2 (SMAD2 产品)

别名

JV18 antibody, JV18-1 antibody, MADH2 antibody, MADR2 antibody, hMAD-2 antibody, hSMAD2 antibody, jv18 antibody, Xmad2 antibody, madh2 antibody, madr2 antibody, XSmad2 antibody, hmad-2 antibody, hsmad2 antibody, jv18-1 antibody, smad-2 antibody, fj43c06 antibody, wu:fj43c06 antibody, 7120426M23Rik antibody, Madh2 antibody, Madr2 antibody, Smad-2 antibody, mMad2 antibody, MAD homolog 2 antibody, Mothers against DPP homolog 2 antibody, SMAD 2 antibody, Smad2 antibody, SMAD family member 2 antibody, SMAD family member 2-Z antibody, SMAD family member 2 S homeolog antibody, SMAD2 antibody, SMAD2Z antibody, smad2 antibody, smad2.S antibody, Smad2 antibody

背景

SMAD2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF?receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8), the common partner, (Smad4) that functions via its interaction to the various Smad's, and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.

分子量

52306

基因ID

4087

NCBI登录号

NP_001003652, NP_005892

UniProt

Q15796

途径

Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus