CD180 抗体 (PE)
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北京 101111
Quick Overview for CD180 抗体 (PE) (ABIN638438)
抗原
See all CD180 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD180 PE
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特异性
- The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
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交叉反应 （详细）
- Human
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纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Human tonsillar B cells
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亚型
- IgG1 kappa
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anti-CD180 Molecule (CD180) (AA 24-220) antibody
CD180 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD180 Molecule (CD180) antibodyCD180 适用: 人 FACS, IP, Func 宿主: 小鼠 Monoclonal G28-8 unconjugated
anti-CD180 Molecule (CD180) antibodyCD180 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal G28-8 unconjugated
anti-CD180 Molecule (CD180) (Internal Region) antibodyCD180 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD180 Molecule (CD180) antibody (APC)CD180 适用: 人 FACS 宿主: 小鼠 Monoclonal G28-8 APC
anti-CD180 Molecule (CD180) antibodyCD180 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD180 Molecule (CD180) (C-Term) antibodyCD180 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-CD180 Molecule (CD180) (N-Term) antibodyCD180 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
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限制
- 仅限研究用
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溶解方式
- No reconstitution is necessary.
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze.
Avoid prolonged exposure to light. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Caspase activity is required for stimulated B lymphocytes to enter the cell cycle." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6065-72, (2003) (PubMed).
: "Activation of human B cells. Comparison of the signal transduced by IL-4 to four different competence signals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 12, pp. 3873-80, (1990) (PubMed).
: "Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4071-8, (1988) (PubMed).
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: "Caspase activity is required for stimulated B lymphocytes to enter the cell cycle." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6065-72, (2003) (PubMed).
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- CD180 (CD180 Molecule (CD180))
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别名
- CD180
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背景
- CD180 Molecule,CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.,LY64, Ly78, RP105, Bgp95
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基因ID
- 4064
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UniProt
- Q99467
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途径
- Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
抗原
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